I tried to keep the profiles spoiler free as the character development plays a very important role in Neon Genesis:Evangelion.
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 Shinji Ikari - The Third Child -Age 14- Shinji is kind of a dry character but almost instantly you sympathize with him.  Who hasn't been alone or scared.  He possesses one of the most prevalant character flaws in the series, lack of self worth.  He is always looking for the admiration and love of his fellow man, but at the same time closes them all off to avoid being hurt.  Contradicting his own needs Shinji is doomed to a lifetime of sorrow.   Unless he can realize his existence is what he wishes it to be and that only he can save himself.

 Rei Ayanami - The First Child - Age 14- Rei is quiet and distant.  She has great importance to Gendou Ikari.  Unadept to living Rei is willing to sacrifice her life at any time.  In the series there are actually three Rei Ayanami's, If you've seen the show you know what I am talking about.  Toward the end Rei starts to show more emotion, especially for Shinji.  There is also a secret behind the dummy plug system involving Rei, I will not say anymore on that, if you want to find out the best way is by watching the series.

 Asuka Langley Soryu - The Second Child - Age 14 - Asuka is loud and brash.  She thinks she is the best there ever was and is quick to let you know about it.  Suffering a childhood of hatred and insanity, Asuka also suffers from a lack of self worth.  Being the best at piloting Eva-02 made up for that and when things get rough her true self appears.  As weak and fragile as Shinji.

 Gendou Ikari - Director of NERV, Gendou is a mystery.  He appears to be a cruel man with selfish desires, but is he?  Father to Shinji, Gendou treats his son as an unwanted burden and is quick to sacrifice his own son or any of the other pilots to keep his plan intact.  Head of not only NERV but also the Human Instrumentality Project, Gendou aspires to create a better life for mankind.  One free of sin and pain, even if it means destroying everyone to achieve it.

 Yui Ikari - Yui is Shinji's mother.  she disappeared while Shinji was still a child and many believed that Gendou had killed her.  In actuality she was absorbed by the Eva unit.  

 Misato Katsuragi - Major Katsuragi is the Director of Operations for NERV.  She is responsible for the battle plans and actions of the Evangelion Units.  A lonely woman with problems of her own she takes Shinji in, in an attempt to fill her lifes own holes.  The lone survivor of the research team at the pole when second impact occured Misato had not spoken for years after the incident.  Eventually her wounds healed and she slipped back into normal life.

 Ritsuko Ikage -

 Toji Suzuhara -  Toji is the typical school jock except that he shows an enormous love for his little sister who has been hospitalized after a battle between Eva-01 and the first angel.  Angry at the pilots carelessness he beats some sense into Shinji after finding out he is that pilot.  He soon sees how difficult it is to be an Eva pilot and apologizes to Shinji,  Toji is soon friends with Shinji.  Selected to be the fourth child and pilot Eva-03 Toji agrees under the condition that his sister is to be transferred to NERV Hospital.

 Kensuke Aida -  Kensuke is a nerd.  There is no other way to put it.  Obsessed with warfare and weapons he is dying to be an Evangelion Pilot.  His father obviously works with the military as Kensuke always has information that he shouldn't regarding the Evangelion and NERV.  A nice guy overall, Kensuke is quick to befriend the new pilot of Eva-01,Shinji Ikari.

 Pen-Pen -  Pen-Pen is a warm water Penguin who loves nothing more than eating and lounging in a hot spring.  According to the manga Misato acquired Pen-Pen from an expiriment facility who no longer needed him.  Pen-pen is greedy, and selfish.

 Maya -

 Prof. Fiyutski -  I have absolutely no idea how to spell this guys name but you get the picture, (Fee-ute-ski).   The professer was Yui's advisor back in college and reluctantly took on Gendou per her request.  A common sense kinda guy he always suspected Gendou had something to do with the second impact and has been with NERV for a long time.  It is difficult to tell but it seems he may have worked for SEELE as a watchdog, keeping an eye on Gendou Ikari.

 Kaji - Misato ex-boyfriend who burst onto the scene with Asuka, Kaji is a double agent.  He is a spy but Gendou knows this and uses him to his own ends.  Kaji is unrefined and unmannered but he knows his way around the Ladies.  Always flirting with the Women of NERV and angering Misato, who he has feelings for but does not show them until its too late.

 Kaoru -  Kaoru Nagisa is technically an angel but he interacts with a main character, therefore making him an important character himself.  Kaoru is an intellectual.  Meaning he delves deep into meaning and tries to bring forth the truth.  He shows extreme kindness to Shinji and befriends him instantly.(Some people think Kaoru is gay, but angels are niether male nor female making homosexuality a moot point).  Born of Adam, Kaoru is capable of bringing forth the third impact but will he decide Humanlife is worthy of existence before it is too late.  I could tell ya but then what fun would that be.

 Hikari -  The class-rep.,Hikari is the typical anime schoolgirl.  Full of life and of course in love with her classmate, Toji Suzuhara.  She plays a very minor part and no real importance to the plot.

 Seele - SEELE is not technically a character, rather a group of charactrers.  Since I do not know their names, they are simply SEELE.  SEELE is a group formed to plan and instrument the scenario brought forth in the Dead Sea Scrolls.  The plan is never explained outright and it is in each viewers perception as to what it actually is(Third Impact?...maybe,watch and figure it out).  Using Gendou, NERV, and the Evangelion to achieve its ultimate goal SEELE grows impatient with Gendou, who they feel is decieving them and trying to betray them.  Thus to speed up the Prophecies of the Dead Sea Scroll they send an angel straight to the one place the Evangelion have been protecting, Terminal Dogma, the resting place of Adam the First Angel.  If contact were to occur between the angel and Adam third impact would destroy existence as prophesized.

 Eva-00 - Unit 00 the prototype is piloted by Rei Ayanami.  In the first Synchronization test Unit-00 went berserk and  nearly killed Rei.  Later it goes berserk a second time with Shinji inside.

 Eva-01 - Unit-01 is pilotedd by Shinji Ikari.  In the first episode Unit-01 activates on its own to protect Shinji.  Throughout the series it activate two more times by itself.  Inhabited by Yui's soul, Eva-01 plays almost just an important role as Shinji.

 Eva-02 - Unit-02 is piloted by Asuka.  Asuka and unit 02 have the highest synch ratio among the Evas at first, but then the soul inside Unit-02 goes into hiding allowing Kaoru to use and manipulate it.